Olfactory receptors are not unique to the nose


https://www.nature.com/articles/d41586-022-01631-0?utm_medium=social&utm_source=facebook&utm_content=ads&utm_term=null&utm_campaign=MLSR_OUTLK_ENGM_GL_MPSS_FIRMN_CF-Smell22&fbclid=IwAR3OSvmhqrLjexXVdx-_mkKHB3p9miC3d3B8Y5i9jAHP1NSpxns-2KK0wJU

Bioink 3-D printing looks promising for damaged ears, nose, and knees


Researchers have created 3-D printed structures out of a special bioink containing human stem cells that encouraged cartilage growth in mousemodels and could be used to make cartilaginous implants for ears, noses, and eventually knees.

“[The implant] had great mechanical properties after 2 months, it looks like cartilage [tissue], and we’ve seen a lot of vascularisation, so blood vessels have grown in between this material. I think this is a huge achievement,” said Dr. Paul Gatenholm, from the Wallenberg Wood Science Center in Sweden who presented his team’s work at the 251st National Meeting & Exposition of the American Chemical Society (ACS), held recently in San Diego, California, US. [ACS 2016, abstract CELL 363]

Gatenholm and his team developed their bioink, called CELLINK, with wood-derived nanofibers mixed with polysaccharides from algae and added human chondrocytes (cells in the cartilage matrix).

Scientists can 3-D bioprint the shape of an ear using human cells that build up cartilage. Photo courtesy of the American Chemical Society.

Scientists can 3-D bioprint the shape of an ear using human cells that build up cartilage. Photo courtesy of the American Chemical Society.

The ketchup-like hydrogel held its shape after 3-D printing into structures such as an ear and produced cartilage. The researchers added human mesenchymal bone marrow stem cells to boost chondrocyte proliferation after implanting the 3-D printed tissue into mice.After 60 days in vivo, the researchers saw chondrocyte proliferation, cartilage production, and a network of blood vessels supporting the cells in the printed structure.

The nanofibers were important to the vascularisation process because they provide structural stability for living cells in a wet environment so that they are contained and have access to oxygen and nutrients flowing into the structure. This differentiates the bioink-printed 3D structures from traditional scaffolding technology, Gatenholm said, on which it can be difficult to seed and contain cells.

“The bioink has very good printing fidelity,” he said. “We can print those fine lines that are crucial for oxygen transport and for nutrient transport…. Another interesting finding was the good mechanical properties of the hydrogel with the printed cells. We implanted it directly after printing, so no incubation in the incubator [was necessary]. Just print [implant] into the animal.”

Though the researchers can print in precise shapes, the cartilage grows in as a flat surface over a couple of months. The short and long term applications would be for plastic surgery, Gatenholm said, after trauma, for example, when pieces of soft tissue like the ear or nose are missing. It might be possible to extend the process to printed spinal disks and later on, knee cartilage, all using the patient’s own cells.

The next step will be larger animal studies and eventually human trials to test the implants’ durability and flexibility, Gatenholm said, as well as different sources of stem cells, such as adipose tissue, which can be retrieved during a 30 minute surgery.

In parallel, he is seeking regulatory approval for the bioink and developing a simple 3-D printer that can run in an operating room

“It’s very simple, it’s like a coffee machine,” Gatenholm said. “Our vision is to do everything in the surgery room.”

Bioink 3-D printing looks promising for damaged ears, nose, and knees


Researchers have created 3-D printed structures out of a special bioink containing human stem cells that encouraged cartilage growth in mousemodels and could be used to make cartilaginous implants for ears, noses, and eventually knees.

“[The implant] had great mechanical properties after 2 months, it looks like cartilage [tissue], and we’ve seen a lot of vascularisation, so blood vessels have grown in between this material. I think this is a huge achievement,” said Dr. Paul Gatenholm, from the Wallenberg Wood Science Center in Sweden who presented his team’s work at the 251st National Meeting & Exposition of the American Chemical Society (ACS), held recently in San Diego, California, US. [ACS 2016, abstract CELL 363]

Gatenholm and his team developed their bioink, called CELLINK, with wood-derived nanofibers mixed with polysaccharides from algae and added human chondrocytes (cells in the cartilage matrix).

Scientists can 3-D bioprint the shape of an ear using human cells that build up cartilage. Photo courtesy of the American Chemical Society.

Scientists can 3-D bioprint the shape of an ear using human cells that build up cartilage. Photo courtesy of the American Chemical Society.

The ketchup-like hydrogel held its shape after 3-D printing into structures such as an ear and produced cartilage. The researchers added human mesenchymal bone marrow stem cells to boost chondrocyte proliferation after implanting the 3-D printed tissue into mice.After 60 days in vivo, the researchers saw chondrocyte proliferation, cartilage production, and a network of blood vessels supporting the cells in the printed structure.

The nanofibers were important to the vascularisation process because they provide structural stability for living cells in a wet environment so that they are contained and have access to oxygen and nutrients flowing into the structure. This differentiates the bioink-printed 3D structures from traditional scaffolding technology, Gatenholm said, on which it can be difficult to seed and contain cells.

“The bioink has very good printing fidelity,” he said. “We can print those fine lines that are crucial for oxygen transport and for nutrient transport…. Another interesting finding was the good mechanical properties of the hydrogel with the printed cells. We implanted it directly after printing, so no incubation in the incubator [was necessary]. Just print [implant] into the animal.”

Though the researchers can print in precise shapes, the cartilage grows in as a flat surface over a couple of months. The short and long term applications would be for plastic surgery, Gatenholm said, after trauma, for example, when pieces of soft tissue like the ear or nose are missing. It might be possible to extend the process to printed spinal disks and later on, knee cartilage, all using the patient’s own cells.

The next step will be larger animal studies and eventually human trials to test the implants’ durability and flexibility, Gatenholm said, as well as different sources of stem cells, such as adipose tissue, which can be retrieved during a 30 minute surgery.

In parallel, he is seeking regulatory approval for the bioink and developing a simple 3-D printer that can run in an operating room

“It’s very simple, it’s like a coffee machine,” Gatenholm said. “Our vision is to do everything in the surgery room.”

In rare disorder, US baby born without a nose


Eli immediately started breathing normally through his mouth. (Photo: AP)

Eli immediately started breathing normally through his mouth.

Mumbai: A woman in Alabama, Brandi McGlathery gave birth to a child who does not have a nose. According to a report in Mobile Press-Register, the newly born was suffering from a rare malformation, known as congenital arhinia which affects 1 in 197 million babies.

According to the mother, Eli immediately started breathing normally through his mouth. Due the complexity of the case, Eli was shifted to USA Children’s and Women’s Hospital in Mobile, Alabama.

Speaking to Mobile Press-Register, Brandi McGlathery said that the doctors performed a tracheotomy to assist with his breathing, and since then he’s been a much happier baby. The mother has to be constantly by his side as Eli does not make a noise while crying.

An article published in 2014 by the U.S. National Library of Medicine states that there had been only 43 recorded cases of congenital arhinia since the first was reported in 1931.

McGlathery told the Press-Register she’s already made contact with parents who have had children born without a nose and other patients who have grown up with the malformation. Due to his current medical condition, the newly born can suffer from breathing and feeding problems in future.

The mother consulted with various doctors on how to take care of the baby at home. Meanwhile, McGlathery told the Press-Register that she and Eli’s father, Troy Thompson, have decided not to do any plastic surgery until he grows up and decides on his own whether to go that route.

“Until the day he wants to have a nose, we don’t want to touch him,” she told the newspaper. “We have to take it day by day.”

Until then, McGlathery said, “he’s perfect the way he is.”

With the news spreading like a fire, family friends set up a GoFundMe page where more than $5,000 have been raised so far.

New study explains why men’s noses are bigger than women’s.


Human noses come in all shapes and sizes. But one feature seems to hold true: Men’s noses are bigger than women’s. A new study from the University of Iowa concludes that men’s noses are about 10 percent larger than female noses, on average, in populations of European descent. The difference, the researchers believe, comes from the sexes’ different builds and energy demands: Males in general have more lean , which requires more oxygen for muscle tissue growth and maintenance. Larger noses mean more oxygen can be breathed in and transported in the blood to supply the muscle.

The researchers also note that males and females begin to show differences in nose size at around age 11, generally, when puberty starts. Physiologically speaking, males begin to grow more lean muscle mass from that time, while females grow more fat mass. Prior research has shown that, during puberty, approximately 95 percent of body weight gain in males comes from fat-free mass, compared to 85 percent in females.

“This relationship has been discussed in the literature, but this is the first study to examine how the size of the nose relates to body size in males and females in a longitudinal study,” says Nathan Holton, assistant professor in the UI College of Dentistry and lead author of the paper, published in the American Journal of Physical Anthropology. “We have shown that as body size increases in males and females during growth, males exhibit a disproportionate increase in nasal size. This follows the same pattern as energetic variables such as oxygenate consumption, basal metabolic rate and daily energy requirements during growth.”

It also explains why our noses are smaller than those of our ancestors, such as the Neanderthals. The reason, the researchers believe, is because our distant lineages had more muscle mass, and so needed larger noses to maintain that muscle. Modern humans have less lean muscle mass, meaning we can get away with smaller noses.

“So, in humans, the nose can become small, because our bodies have smaller oxygen requirements than we see in archaic humans,” Holton says, noting also that the rib cages and lungs are smaller in modern humans, reinforcing the idea that we don’t need as much oxygen to feed our frames as our ancestors. “This all tells us physiologically how have changed from their ancestors.”

Holton and his team tracked nose size and growth of 38 individuals of European descent enrolled in the Iowa Facial Growth Study from three years of age until the mid-twenties, taking external and internal measurements at regular intervals for each individual. The researchers found that boys and girls have the same nose size, generally speaking, from birth until puberty percolated, around age 11. From that point onward, the size difference grew more pronounced, the measurements showed.

“Even if the is the same,” Holton says, “males have larger noses, because more of the body is made up of that expensive tissue. And, it’s at puberty that these differences really take off.”

https://i0.wp.com/cdn.physorg.com/newman/gfx/news/2013/thebigmaleno.jpg

Holton says the findings should hold true for other populations, as differences in male and female physiology cut across cultures and races, although further studies would need to confirm that.

Prior research appears to support Holton’s findings. In a 1999 study published in the European Journal of Nutrition, researchers documented that males’ energy needs doubles that of females post-, “indicating a disproportional increase in energy expenditure in during this developmental period,” Holton and his colleagues write.

Another interesting aspect of the research is what it all means for how we think of the nose. It’s not just a centrally located adornment on our face; it’s more a valuable extension of our lungs.

“So, in that sense, we can think of it as being independent of the skull, and more closely tied with non-cranial aspects of anatomy,” Holton says.

Endoscopic endonasal approach to cholesterol granulomas of the petrous apex: a series of 17 patients


The aim of this study was to report the results in a consecutive series of patients who had undergone an endoscopic endonasal approach (EEA) for drainage of a petrous apex cholesterol granuloma (CG).

Methods

Seventeen cases with a confirmed diagnosis of petrous apex CG were identified from a database of more than 1600 patients who had undergone an EEA to skull base lesions at the authors’ institution in the period from 1998 to 2011. Clinical outcomes were reviewed and compared with those in previous studies of open approaches.

Results

Nine patients underwent a transclival approach and 8 patients underwent a combined transclival and infrapetrous approach. A Silastic stent was used in 11 patients (65%), a miniflap in 4 (24%), and a simple marsupialization of the cyst in 3 (18%). All symptomatic patients had partial or complete improvement of their symptoms postoperatively and at the follow-up (mean follow-up 20 months, range 3–67 months). Complications developed in 3 patients (18%) including epistaxis, chronic serous otitis media, eye dryness, and a transient sixth cranial nerve palsy. Two patients (12%) had a symptomatic recurrence of the cyst requiring repeat endoscopic endonasal drainage. There were no instances of internal carotid artery injuries, CSF leaks, or new hearing loss. The mean postoperative hospital stay was 2 days (range 0.7–4.6 days). These results were comparable with those in previous studies of open approaches to petrous apex CGs.

There was a strong correlation between the size of the cyst and the type of approach chosen (Rpb [point biserial correlation coefficient] = +0.67, p = 0.003359) and a very strong correlation between the degree of medial extension (defined by the V-angle) and the choice of approach (Rpb = +0.81, p < 0.0001). Based on these observations, the authors developed an algorithm for guiding the choice of the most appropriate route of drainage.

Conclusions

The EEA is a safe and effective alternative to traditional open approaches to petrous apex CGs.

Source: Journal of Neurosurgery